ANTIMICROBIAL ACTIVITY OF THE EXTRACTS FROM ALBIZIA LEBBECK

Abstract

The objective of this research was to study the extraction techniques and antimicrobial activity of crude extracts of Albizia lebbeck, with parts of barks and roots by the extraction method using Hexane, Dichloromethane, Ethyl Acetate and Methanol as solvents. The crude extracts were obtained from Agar disc-diffusion method and tested for resistance against 9 species of microorganisms: Staphylococcus aureus, Bacillus cereus, Bacillus thuringiensis, Escherichia coli, Enterobacter aerogenes, Salmonella typhimurium, Aspergillus niger, Candida albicans and Saccharomyces cerevisiae of clear zone diameter.  The results found that the percentage yield (%) of crude extracts from barks was higher than the root extracts from the Albiza lebbeck. The optimum extract concentration in the antimicrobial activity test was 0.1% m/v. The Ethyl Acetate crude extract from the barks and roots had antimicrobial activities. The Ethyl Acetate crude extract from the bark of Staphylococcus aureus was the best at the level of 11.08 mm. The most effective resistance against Bacillus cereus was 12.66 mm in Gram-negative bacteria. The crude extract from the roots was the best in Escherichia coli at 10.00 mm, but no gram-negative antibacterial was found in the crude extract. The Hexane crude extract had the best antifungal effect, which could resist Saccharomyces cerevisiae the best. The crude extract from the barks had the resistance level at 8.30 mm. The crude extract from the roots had the resistance level at 10.08 mm. The crude extract from the roots of Albizia lebbeck had the resistance level of 10.80 mm. The Albizia lebbeck could be extracted by the solvent extraction method and crude extracts from the roots of Albizia lebbeck, and the barks had better antimicrobial activities than the roots.